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opal 520  (Akoya Biosciences)


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    Structured Review

    Akoya Biosciences opal 520
    Opal 520, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 1274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opal+520/Opal+480/pmc13276796-93-5-9
    Average 99 stars, based on 1274 article reviews
    opal 520 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Incubation:

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Article Title: Neurochemical phenotype of relaxin family peptide receptor-3 (RXFP3) lateral hypothalamus/zona incerta cells
    Article Snippet: .. Sections were then incubated in HRP C1 (15 minutes), Opal 520 (Akoya Biosciences, #FP1487001KT; 1:750; 30 minutes), and then HRP blocker (15 minutes) to develop the fluorophore signal for the probe assigned to channel 1. .. The same procedure was repeated for HRP C2 and HRP C3, using Opal 570 (Akoya Biosciences, #FP1488022KT; 1:750) and Opal 690 (Akoya Biosciences, #FP1497001KT; 1:750) as fluorophores, respectively.

    Blocking Assay:

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Amplification:

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Expressing:

    Article Title: Distribution of Metabotropic Serotonin Receptors in GABAergic and Glutamatergic Neurons in the Auditory Midbrain
    Article Snippet: .. After that, opal dyes (1:1000) were assigned for each channel: Vgat and Vglut2 expression were identified by Opal 520 (Akoya Bioscience, catalog #FP1487001KT), serotonin receptors were identified by either Opal 570 (Akoya Bioscience, catalog #FP1488001KT) or 690 (Akoya Bioscience, catalog #FP1497001KT). .. Following staining with DAPI, slices were coverslipped using ProLong Gold Antifade Mountant (Fisher Scientific, catalog # P36934 ).

    Fluorescence:

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), https://doi.org/10.3390/cells15050432 Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Imaging:

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), https://doi.org/10.3390/cells15050432 Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Staining:

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), https://doi.org/10.3390/cells15050432 Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    other:

    Article Title: Lineage tracing reveals photoreceptor precursor cell subpopulations that contribute to murine retinogenesis
    Article Snippet: Images were processed and quantified in FIJI (ImageJ, version 1.54f) ( ).



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